addGenesPerc

addGenesPerc

Description

calculates the total percentage of (normalized) counts for a subset of selected genes

Usage

addGenesPerc(
  gobject,
  expression_values = c("normalized", "scaled", "custom"),
  genes = NULL,
  vector_name = "gene_perc",
  return_gobject = TRUE
)

Arguments

Argument

Description

gobject

giotto object

expression_values

expression values to use

genes

vector of selected genes

vector_name

column name as seen in pDataDT()

return_gobject

boolean: return giotto object (default = TRUE)

Value

giotto object if return_gobject = TRUE, else a vector with % results

Examples

data(mini_giotto_single_cell)

# select genes (e.g. Rpl or mitochondrial)
random_genes = sample(slot(mini_giotto_single_cell, 'gene_ID'), 5)

# calculate percentage of those selected genes per cells/spot
updated_giotto_object = addGenesPerc(mini_giotto_single_cell,
genes = random_genes,
vector_name = 'random_gene_perc')

# visualize result in data.table format
pDataDT(updated_giotto_object)